Supplementary Figure 7: ago1 D580A and ago1 F276A R773E are dominant-negative alleles when overexpressed.
From: Small-RNA loading licenses Argonaute for assembly into a transcriptional silencing complex
Tenfold serial dilutions of otr1R::ura4+ pericentromeric silencing reporter cells of the indicated genotypes, transformed with an empty vector or a 3xFLAG-ago1 overexpression plasmid (wild-type or mutant as noted), plated on non-selective medium or medium containing 5-FOA.
