Supplementary Figure 3: Summary of constitutive activity of GPCR-Tango constructs used in this study.
From: PRESTO-Tango as an open-source resource for interrogation of the druggable human GPCRome
HTLA cells were transfected with various Tango constructs, plated into 384-well assay plates, and luminescence in relative luminescence units (RLU) was measured after overnight incubation in the absence of ligand. The constitutive activity of these constructs, i.e., the ratio of the maximum to the minimum luminescence of the constructs, varied over a range of up to 551-fold in individual experiments.
