Supplementary Figure 3: TMRM labeling of WT and cysteine mutants of LmECF–RibU.
From: ATP binding drives substrate capture in an ECF transporter by a release-and-catch mechanism
(a) Ribbon diagram of the LmECF–RibU homology model with each subunit colored as indicated and cysteine residues shown as red spheres. The position of the membrane is represented as black lines. (b) Purified LmECF–RibU variants were labeled with the thiol reactive probe, TMRM, for the indicated time points. After SDS-PAGE (top panels), the labeling of the individual subunits was visualized on a fluorescence scanner (bottom panels). (c) Initial rates of TMRM labeling were calculated from these data.
