Supplementary Figure 4: AMP-PNP is a low-affinity competitive inhibitor of LmECF–RibU.
From: ATP binding drives substrate capture in an ECF transporter by a release-and-catch mechanism
(a) ATPase activity of LmECF–RibU in the presence of increasing concentrations of AMPPNP. Analytical SEC traces of LmECF–RibU measured in the presence of the vitamin B2 transport substrate and several concentrations of AMPPNP detected by UV absorbance (b) or riboflavin fluorescence (c).
