Supplementary Figure 2: TMRM labeling of WT and cysteine mutants of LmRibU.
From: ATP binding drives substrate capture in an ECF transporter by a release-and-catch mechanism
(a) Purified LmRibU variants were incubated with the thiol reactive probe, TMRM, for the indicated time points. After termination of the reaction by DTT, samples were subjected to SDS-PAGE and TMRM labeling was visualized on a fluorescence scanner. (b) Initial rates of TMRM labeling were calculated from the data shown in (a).
