Supplementary Figure 2: Comparison of the active site of the Ubc13~Ub–RING complexes with and without Ube2V2 and the UbcH5A~Ub–RING complex (4AP4).
From: Structural basis for the RING-catalyzed synthesis of K63-linked ubiquitin chains
E2’s from the Ubc13~Ub–RING complexes with and without Ube2V2 and the UbcH5A~Ub–RING complex (4AP4) (Plechanovová, A. et al., Nature. 489, 115–20, 2012) were superimposed. Ubc13, Ub and RING-RING from the Ubc13~Ub–RING complex are coloured in pale green, pale orange and pale pink respectively. UbcH5A, Ub and RING-RING from the UbcH5A~Ub–RING complex are coloured in teal, grey and purple respectively. Ube2V2, Ubc13, Ub and RING-RING from the Ube2V2–Ubc13~Ub–RING complex are coloured in blue, dark green, yellow and dark pink respectively. Ub* is coloured in orange.
(a) Molecular detail of the active site of the Ubc13~Ub–RING complex and the UbcH5A~Ub–RING complex where the E2’s are superimposed.
(b) Molecular detail of the active site of the Ube2V2–Ubc13~Ub–RING complex and the Ubc13~Ub–RING complex where Ubc13 from each complex is superimposed.
