Supplementary Figure 5: Raw data for ITC experiments for the Ubc13-Ube2V2 interaction.
From: Structural basis for the RING-catalyzed synthesis of K63-linked ubiquitin chains
ITC experimental data was collected for the WT Ubc13–Ube2V2 interaction and mutants.
(a) The top panel for each experiment shows the raw data (black line) and the background fitting (red line). Data were fitted using MicroCal Origin software. The bottom panel for each experiment shows integrated heats of injection (black squares) and the best fit using a one binding site model (black line).
(b) ITC derived Kd measurements (± error) of Ube2V2–Ubc13 interaction for WT and mutants.
