Supplementary Figure 5: hIntL-1 exhibits specificity for microbial glycan epitopes bearing terminal 1,2-diols.

(a) hIntL-1 binding to immobilized α-Neu5Ac assayed by the ELISA-like carbohydrate-binding assay (Supplementary Fig. 1c). Data are fit to a one site binding equation (solid lines). Data are presented as the mean (n=2 of a technical replicate and is representative of three independent experiments).

(b) Inhibition of hIntL-1 binding to immobilized β-Galf. Four compounds (glycerol, 1-phosphoglycerol, the methyl-α-glycoside of Neu5Ac, and the methyl-α-D-mannopyranoside) were dissolved in binding buffer and included during the hIntL-1 incubation. Binding data shown are relative to a control where no competitor was added to the binding buffer. Data are presented as the mean (n=2 of a technical replicate and is representative of three independent experiments).