Supplementary Figure 7: ASF1a colocalizes with MCM2 and CDC45.
From: A unique binding mode enables MCM2 to chaperone histones H3–H4 at replication forks
(a –c) PLA of endogenous chromatin-bound ASF1a with MCM2 (panels a, c) and CDC45 (panel b) as described in Fig. 6 b–d. Images showed a large field of pre-extracted cells to complement the single cells shown in Fig. 6. Scale bars were 16 μm. (d) Interactions of MCM2 HBD and H3.2–H4 in the MCM2 HBD–H3.2–H4dimer–ASF1 complex, highlighting the region S2 as in Fig. 1e. (e) Alignment of the MCM2 HBD binding region in histone H3 variants, showing strict conservation in red and conservation of properties in green. (f) Superposition of the H3–H4 tetramer from the MCM2 HBD–H3–H4 tetramer structure (in color) and the CENPA–H4 tetramer from the CENPA nucleosome structure (in silver).
