Supplementary Figure 8: Biochemical characterizations of the MCM2–CENPA–H4 tetramer complex.
From: A unique binding mode enables MCM2 to chaperone histones H3–H4 at replication forks
(a) Gel-filtration analyses of the CENPA–H4 tetramer and MCM2 HBD(43–160)–CENPA–H4 tetramer complex (complex 7) in high salt buffer (1 M NaCl). (b) The peak fractions from panel a were analyzed by SDS-PAGE. (c) The same analyses as in panel a, but with a low salt buffer (0.3 M NaCl). (d, e) IP of Flag-HA-MCM2 WT and its H3–H4-binding mutant from cells stably expressing EGFP-CENPA (Amaro, A.C. et al., Nat Cell Biol 12, 319-329, 2010).
