Supplementary Figure 4: List of 32 hexapeptides derived from the peptide-library hits used to generate the sequon for Figure 4.

Experimentally determined O-GlcNAc sites were analyzed for sequence similarities. Peptides were aligned by the modified serine or threonine, and the sequence truncated to include only 3 residues to the N-terminus of the GlcNAc site (“−3”) and 2 residues to the C-terminus of the site (“+2”) as dictated by the ordered binding of peptides in only these subsites from the structural data. The hexapeptides highlighted in yellow were used for the generation of the sequence logo shown in Fig. 4b.