Supplementary Figure 5: Binding of TNT-mutant proteins to the antitoxin IFT.
From: The tuberculosis necrotizing toxin kills macrophages by hydrolyzing NAD
The purified mutant proteins TNTQ822A (a) or TNTH792N Q822K (b) were used in an in vitro pull-down assay with purified MBP-IFT. MBP-IFT alone bound amylose resin while the TNTQ822A or TNTH792N Q822K proteins did not. Incubation of MBP-IFT (1 µg) with equimolar amounts of TNTQ822A or TNTH792N Q822K proteins resulted in binding of both TNT proteins to amylose resin and elution with maltose.
