Supplementary Figure 8: Plasmid construction for this study.
From: The tuberculosis necrotizing toxin kills macrophages by hydrolyzing NAD
Plasmid names are shown in dark grey boxes. The oligonucleotide pairs used for PCR amplification and restriction enzymes utilized for cloning are listed in white boxes. The template DNA for PCR is indicated below the oligonucleotide pairs. When chromosomal DNA was used as a template, the originating organism is indicated.
