Supplementary Figure 2: Expression and purification of TNT–IFT complex. | Nature Structural & Molecular Biology

Supplementary Figure 2: Expression and purification of TNT–IFT complex.

From: The tuberculosis necrotizing toxin kills macrophages by hydrolyzing NAD

Supplementary Figure 2

(a) Schematic flow chart of the purification procedure with accompanying yield at various steps. (b) Fractions were taken from each step of the purification process and analyzed in Coomassie-stained SDS-polyacrylamide gels. The final protein complex after gel filtration chromatography (pooled fractions) was used for X-ray crystallization. (c) Fractions from purification of free TNT were analyzed by Coomassie staining. TNT was separated from the TNT-IFT complex by heating at 70°C for 10 min. TNT was recovered from the soluble fraction while IFT remained in the precipitate after heating. WCL, whole cell lysate; SN, soluble.

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