Supplementary Figure 4: Packing of Rab11–FIP3–Rabin8C complexes in the crystals.
From: Structure of Rab11–FIP3–Rabin8 reveals simultaneous binding of FIP3 and Rabin8 effectors to Rab11
(a) Crystal packing for the 3.0Å P21 data reveals oligomerization of Rab11- FIP3RBD-Rabin8C dodecamers via the well-characterized FIP3RBD homo-dimerization interface. (b) Structure of dodecameric Rab11-FIP3RBD-Rabin8C as found in the asymmetric unit of the crystals. In this structure, two central FIP3RBD helices dimerize to form an asymmetric dodecamer with close contacts to only one of the two Rabin8C dimers. The contacts between the central FIP3RBD homo-dimer and the 1st Rabin8C homo-dimer induce a rotation that results in close interactions of the 3rd and 4th Rab11 molecule. This in turn exposes one FIP3RBD helix at each side of the dodecamer as shown in the perpendicular view.
