Supplementary Figure 2: Molecular replacement with cryo-EM 3D reconstruction for determining the crystal structure of TLR13–ssRNA13.
From: Structural basis for specific recognition of single-stranded RNA by Toll-like receptor 13
(a) Plot of figure-of-merit weighted phase errors (wMPE) calculated relative to the subsequently refined structure versus resolution for the various steps of phase improvement, and maps of the same region of the structure calculated with (b) phases from Cryo-EM potential map based molecular replacement solution (MapCC 0.33). Electron density (MapCC 0.55) after 1 cycle (c) and 5 cycles (d) of density modification (MapCC 0.77). The backbone of the final model is displayed in (b), (c) and (d).
