Supplementary Figure 4: STAS-domain structure.
From: Structure of a prokaryotic fumarate transporter reveals the architecture of the SLC26 family
(a) Ribbon representation of the STAS domain of SLC26Dg with secondary structure elements labeled. (b) Superposition of Cα-traces of the SLC26Dg structure (red) with the equivalent domain from the SLC26 transporter from Rhodobacter sphaeroides (SLC26Rs, blue, PDB code 3oiz), which shares 50% of identical residues. (c) Superposition of the SLC26Dg STAS domain on a structure of the equivalent domain of rat Prestin that lacks a long unstructured loop connecting α1 and β312 (SLC26A5, blue, PDB code 3llo). (d) Superposition of the SLC26Dg STAS domain on the STAS domain ACP complex of the E. coli transporter DauA11 (PDB code 3ny7). The DauA STAS domain is colored in blue, ACP in green. ACP binds to a region on the C-terminus of the STAS domain of DauA that is not defined in the electron density of SLC26Dg. The binding interface does not overlap with the nanobody-binding epitope on the STAS domain of SLC26Dg.
