Supplementary Figure 3: Electron density of the SLC26Dg–Nb5776 complex.
From: Structure of a prokaryotic fumarate transporter reveals the architecture of the SLC26 family
Proteins are shown as sticks with carbons colored in green. 2Fo-Fc density (calculated at 3.2 Å, sharpened with b=90 and contoured at 1σ) calculated with phases obtained from the refined model is superimposed on the structure. Sections of different regions of the complex are shown: (a) Stereo view of the substrate binding site of the transmembrane domain with a bound DM molecule (with carbon atoms of the detergent colored in blue). The detergent binds to a similar location as a bound detergent in the UraA structure. (b) STAS domain viewed from the nanobody binding interface with α-helices 2 and 3 labeled. (c) View of the nanobody. The electron density for the nanobody is less well defined than the density of the transmembrane domain of SLC26Dg, which indicates a higher degree of disorder. The entire nanobody is shown. Supplementary Figs. 3, 4, 5, 6 were prepared with DINO (http://www.dino3d.org).
