Supplementary Figure 7: Cryo-EM analysis of stabilized FMDV particles.
From: Structure-based energetics of protein interfaces guides foot-and-mouth disease virus vaccine design
Purified inactivated O1M and SAT2 S93Y particles were used for data collection by cryoEM. (a) and (b) Representative aligned average (motion corrected) image of O1M VP2 S93Y particles and the corresponding Fourier transform. (c) and (d) Representative aligned average (motion corrected) image of SAT2 VP2 S93Y particles and the corresponding Fourier transform. (e) and (f) FSC curves of the final 3D reconstruction obtained using gold-standard refinement using RELION, marked with the resolution corresponding to a Fourier shell correlation (FSC) of 0.5 and 0.143. The scale bar indicates 100nm.
