Supplementary Figure 6: Point and truncation mutations of HflX affect the 70S-spliting activity of HflX.
From: HflX is a ribosome-splitting factor rescuing stalled ribosomes under stress conditions
(a) The splitting of vacant 70S ribosomes, with two truncation mutations of HflX (in the presence of GMPPNP) determined by SDGC, with a comparison to wild type HflX. (b) HflX double point mutations (R49A-K50A, K55A-K62A, R164A-R165A, R168A-R170A, R185A-R189A, R192A-K194A) significantly impairs the 70S-splitting activity. (c) Binding of mutant forms of HflX to the 50S subunits in the presence of GMPPNP examined using co-sedimentation assay. (d) Control experiments for (c) run without 50S subunits. Bands of HflX are indicated by asterisks.
