Supplementary Figure 2: Heat map of protein abundance as a function of transcript length for ribosome-assembly factors for which clear stage-specific assignments could be made.

Replicate 1 (identical sample as shown in Fig. 2b) and replicate 2 (a second independent experiment) are shown. Iog2 iBAQ values for relevant proteins identified in each construct were used to generate a heat-map of SSU processome assembly dynamics, where low abundance (<20) is shown in gray ranging to high abundance (>30) shown in red. Undetected signal is shown in black. Proteins are grouped into functional units, corresponding to the model presented in Figure 3. PolyA binding protein and streptavidin are shown at the top to represent internal controls. Proteins for which no clear stage-specific assignment could be made are listed in Supplementary Table 1 together with all original mass spectrometry data.