Supplementary Figure 3: Specificity of phospho-Shc pY239/240–specific antibody.
From: EGF-receptor specificity for phosphotyrosine-primed substrates provides signal integration with Src
Serial dilutions of peptides corresponding to the sequence surrounding Tyr239 of Shc1 were spotted on nitrocellulose and probed with a phospho-Shc pY239/240 antibody (Cell Signaling Technology Cat #2434; PDHQYYNDAKKK = Y-Y; PDHQYpYNDAKKK = Y-pY; PDHQpYYNDAKKK = pY-Y; PDHQpYpYNDAKKK = pY-pY). 40nmol corresponds to 2μL of a 20mM stock solution of each peptide (20mM is the solubility limit of the peptides in Tris buffer).
