Supplementary Figure 6: Bromolipids.
1H NMR spectrum of 1,2-di-(9,10-dibromo)stearoyl-sn-glycero-3-phosphocholine. The sample was solved in CDCl3 and the spectrum recorded at room temperature on a 400 MHz Bruker Avance III spectrometer: CDCl3): δH 0.91 (6H, m, ω-CH3), 1.30 (40H, m, CH2), 1.61 (7H, m, β-CH2 and H2O), 1.87 (5H, m, trans CH2CHBr), 2.06 (3H, m, gauche CH2CHBr), 2.32 (4H, m, α-CH2), 3.47 (9H, s, N(CH3)3), 3.54 (4H, m, CHBr), 4.06 (2H, m, PO3CH2 (glycerol)), 4.16 (1H, m, CH2O (glycerol)), 4.22 (2H, m, CH2N), 4.39 (1H, m, CH2O (glycerol)), 4.48 (2H, m, CH2O (glycerol)), 5.24 (1H, m, CHO)5,6. The sample was also examined with positive ion electrospray mass spectroscopy in the presence of formic acid with an Agilent Technologies 6120 ESI-MS. Expected highest peak for (M+H)+ = 1106.3 m/z (observed 1106.2 m/z). The peak pattern was as expected for an isotope cluster with four bromine atoms. The highest intensity peaks cluster in the experimental spectrum can be explained with the (M-Br) fragment. The peak pattern was as expected for an isotope cluster with three bromine atoms. Isotope clusters were simulated with the program IsoPro 3.1 (Mike Senko; https://sites.google.com/site/isoproms/).
