Supplementary Figure 5: ChIP-qPCR validation of changes in factor binding upon Rnaseh1 overexpression.
From: R loops regulate promoter-proximal chromatin architecture and cellular differentiation
a-b, ChIP-qPCR measurements of Tip60 (a) or Suz12 (b) association with genes that harbor minimal, modest, or high levels of R-loops, and the effect of R-loop destruction by Rnaseh1 overexpression are shown. Error bars, s.d. (n = 3 technical replicates from one of two independent cell cultures for each line.) P values comparing Rnaseh1 overexpression to controls were calculated using two tailed students t-tests (*P <0.05; **P < 0.01).
