Supplementary Figure 2: Acute transcription inhibition inhibits Tip60–p400 binding to chromatin.
From: R loops regulate promoter-proximal chromatin architecture and cellular differentiation
a, Western blots of indicated proteins showing unchanged levels of Tip60–p400 subunits p400, Hdac6, Dmap1, Tip60, and Ruvbl1 upon DRB or Triptolide treatment. Actin serves as a loading control. b, Transcript levels of rapidly-degraded transcripts (Nanog, Myc) and slowly-degraded transcripts (Oct4, Gapdh), measured by RT-qPCR. Levels in cells treated with indicated transcription inhibitors are expressed relative to control cells. Error bars, s.d. (n = 3 technical replicates from one of two independent cell cultures for each condition.) c, ChIP-qPCR of the p400 subunit of Tip60–p400 complex with or without transcription inhibitor treatment. IgG serves as a negative control for ChIP. Enrichment levels are expressed for each locus as a fraction of input. Error bars, s.d. (n = 3 technical replicates from one of two independent cell cultures for each condition.) P values were calculated using two tailed students t-tests (*P < 0.05; **P < 0.01).
