Supplementary Figure 5: Association of Pfk2 and Eno1 with mRNAs is not ribosome dependent.
From: Conserved mRNA-binding proteomes in eukaryotic organisms
Reverse transcription (RT)-PCR with gene specific primers to detect mRNAs in affinity isolates of indicated TAP-tagged proteins (Shg1, Pfk2 and Eno1) in the presence or absence of 1 mM puromycin (Puro). Shg1-TAP was used as a positive control to assess the efficiency of puromycin treatment to relieve co-translational association of SET1 mRNA with the SET1C complex, as shown previously (Halbach, A. et al., EMBO J. 28, 2959-70, 2009). Untagged cells (BY4741) served as a negative control (Ctrl). The input refers to total RNA from non-UV crosslinked cells. Products were visualized on an agarose gel.
