Supplementary Figure 3: Activity and stability of cysteine mutants.
From: The bacterial dicarboxylate transporter VcINDY uses a two-domain elevator-type mechanism
(a) Initial rates of 3H-succinate transport by wild type VcINDY (VcINDYwt) and cysteine-free VcINDY (Cysless) after reconstitution into liposomes. Triplicate datasets were used and error bars represent S.E.M. (b) Size exclusion chromatography (SEC) traces showing the normalized absorbance at A280 for Cysless and the 4 double cysteine mutants; A120C V165C (red), A346C V364C (yellow), T154C V272C (brown), and L60C S381C (light blue). V0 is ~8 ml and the VcINDY elution peak is centered on 12.5 ml on the S200 SEC column.
