Supplementary Figure 5: Chemical cross-linking with CuPhen protects outward-stabilizing cysteine pairs from mPEG5K labeling.
From: The bacterial dicarboxylate transporter VcINDY uses a two-domain elevator-type mechanism
SDS-PAGE band-shift assay showing the number of free cysteines present in Cysless and the three double cysteine mutants; A120C V165C, T154C V272C, andA346C V364C, with (+) and without (-) prior treatment with CuPhen. The following protein species seen in the gels are indicated by colored arrows; unmodified VcINDY (red arrow), dimeric VcINDY (orange arrow), singly PEGylated VcINDY (blue arrow), and doubly PEGylated VcINDY (magenta arrow). This experiment was repeated twice with the same outcome.
