Supplementary Figure 5: Estimation of fraction of FliG in an extended conformation in different NaCl concentrations.
From: Domain-swap polymerization drives the self-assembly of the bacterial flagellar motor
Background-subtracted SAXS scattering (left panels) and corresponding interatomic distance distributions (right panels) from FliGMC collected in 150 mM (A and B), 250 mM (C and D), 500 mM (E and F) and 1000 mM NaCl (G and H) with experimental data in black, green, blue and red for each NaCl concentration respectively. Theoretical scattering for the compact and extended conformation are shown as dashed and dotted lines respectively. Grey curves are generated from a linear combination of these theoretical scattering curves: Ifit(q)= f x Icompact(q) + (1-f) x Iextended(q) where f represents the fractional contribution from the compact monomer. The fit was optimised by minimising the root mean squared deviation from the experimental scattering curve. (I) shows a plot of normalized RMSD vs f in each NaCl concentration The best fits correspond to a fractional population of the extended conformation of 0.48, 0.38, 0.07 and 0.01 for NaCl concentrations of 150 mM, 250 mM, 500 mM and 1000 mM NaCl respectively.
