Supplementary Figure 3: Strand β1 of the BamA β-barrel might participate in β-augmentation. | Nature Structural & Molecular Biology

Supplementary Figure 3: Strand β1 of the BamA β-barrel might participate in β-augmentation.

From: Structure of the BAM complex and its implications for biogenesis of outer-membrane proteins

Supplementary Figure 3

Residues T243, S324, D399 (or T400) and G429 of BamA are located at the middle point of a β strand of POTRA3, POTRA4, POTRA5 and strand β1 of BamA β barrel, respectively. These β strands are located within the chamber of the BAM complex structure and have a potential role in participating β-augmentation for OMP substrates. a. G429P mutant was lethal to the bacteria while other mutants were not. b. G429P mutant was lethal in the presence of 10 μM IPTG. c. Western blot showing that G429P mutant protein was expressed in a similar level to that of the wild-type in the cell. d. Purified G429P mutant protein was well-folded. Purified G429P protein from membranes exhibited apparently heat-modifiable mobility on a 15% SDS-PAGE gel.

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