Supplementary Figure 4: PEGylation of FLN5 Y719E RNCs.
From: A structural ensemble of a ribosome–nascent chain complex during cotranslational protein folding
(a) Autoradiography of SDS-PAGE gels as presented in a cropped form in Fig. 4b is provided here for clarity. The arrows indicate the different forms of FLN5 nascent chains observed: tRNA-bound nascent chain (green), PEGylated tRNA-bound nascent chain (red) and released nascent chain (cyan), PEGylated released nascent chain (blue). Approximate migration of molecular weight standards is indicated on the left. (b) RNase A treated FLN5 Y719E RNCs, showing the PEGylation achievable on the released nascent chains (color coding as in a). Approximate migration of molecular weight standards is indicated on the left.
