Supplementary Figure 7: Interactions between folded and unfolded isolated FLN5 variants with 70S ribosomes.
From: A structural ensemble of a ribosome–nascent chain complex during cotranslational protein folding
Relative peak intensities of: (a) FLN5 in the presence of 1 molar equivalent of 70S ribosomes relative to FLN5 alone, at 5 μM (as presented in Fig. 6b) (b) FLN5Δ12 in the presence of 1 molar equivalent of 70S ribosomes relative to FLN5Δ12 alone, at 5 μM. (c) FLN5 Y719E in the presence of 1 molar equivalent of 70S ribosomes relative to FLN5 Y719E alone, at 8 µM (as presented in Fig. 6b). Shaded areas highlight the peak broadenings observed.
