Supplementary Figure 3: Mutant hTR localizes to cyTER bodies in U2OS cells.
From: Inhibition of telomerase RNA decay rescues telomerase deficiency caused by dyskerin or PARN defects
Mutant A377G or C408G hTR is mislocalized to the cytoplasm in cyTER bodies in U2OS cells. a, Cytoplasmic localization of A377G or C408G RNA (white arrowheads) by FISH in U2OS cells. The nucleus is labeled with an antibody that recognizes either of the nuclear envelope components lamin A or lamin C (Scale bar 5 μm). White numbers in Merge, % of cells showing hTR localization to Cajal bodies (mean+/-s.d., n=4 independent experiments). b, Cytoplasmic localization of C408G hTR by FISH (nucleus outlined with lamin A/C staining, cell outlined with pan cadherin staining) (Scale bar 5 μm). White numbers in Merge, % of cells showing hTR cytoplasmic localization (mean+/-s.d., n=4 independent experiments).
