Supplementary Figure 2: Alignment of full-length YL1 and stereo view of the elongated αC helix.
From: Molecular basis and specificity of H2A.Z–H2B recognition and deposition by the histone chaperone YL1
a) Sequences of YL1 proteins from different organisms have been aligned. For clarity, fungi sequences have not been included since they are twice as long as their other homologs. See alignment of the YL1–ZID region in Supplementary Figure 1f for incorporation of the fungi sequences. Conservation is shown with different hues of blue (strong, dark blue; weak, light blue). Red boxes highlight the three main regions conserved throughout evolution (fungi display similar conservation patterns as those shown on this figure). Numbering above the sequences corresponds to the human protein.
b) Stereo view of the Fo–Fc simulated-annealing omit map contoured at 2.5 σ of H2A.Z elongated αC helix in the YL1–ZID–H2A.Z–H2B complex. Residues spanning the end of the canonical plus the extended part of the αC helix were removed prior to simulated-annealing.
