Supplementary Figure 5: Effect of yeast Swc2-Z regional mutations on H2A.Z exchange activity and H2A.Z occupancy.
From: Structural basis of H2A.Z recognition by SRCAP chromatin-remodeling subunit YL1
(a), Schematic view of Swc2-Z(YL1-Z) family and Mut2 regions subject to mutagenesis. Residues substituted with alanine are labeled "A". (b), Phenotypes of WT, swc2Δ, swc2-ΔZ (Swc2 residues 1–89 are deleted) strains. (c), ChIP-PCR for H2A.Z-HA occupancy in yeast strains containing Swc2 WT, Swc2-Mut2 and Swc2-ΔZ, in which Swc2 residues 1–89 are deleted. (d), Histone exchange assay for SWR1 WT, SWR1 (Swc2 Mut2) and SWR1 (Swc2-ΔZ). The reaction mix contained 5 nM nucleosome, 2 nM SWR1, 5 nM H2A.Z 3×Flag–H2B dimer and 1 mM ATP.
