Supplementary Figure 7: Aggregon mutation restores the ability of Drosophila Axin V72R to assemble in punctate cytosolic complexes.
From: Axin cancer mutants form nanoaggregates to rewire the Wnt signaling network
(a) Confocal microscopy analysis of human Axin (hAxin)-wt, hAxin-L106R and hAxin-L106R F119R in HEK293T cells. Scale bars 10 μm. (b) Confocal microscopy analysis of Drosophila Axin (DAxin)-wt, DAxin-V72R, DAxin-V72R F87R (homologue of human aggregon suppressor), DAxin-V72R I169R L170R (Drosophila aggregon suppressor) in HEK293T cells. Note that the Drosophila aggregation suppressor mutation (I169R L170R) reverts the protein into puncta while the potential suppressor mutation at the position of the human aggregon (F87R) fails to do so. Scale bars 10 μm. Schematic RGS domain structures indicate the location of cancer (red) and aggregon (green) mutations.
