Supplementary Figure 2: Characterization of Axin L106R aggresome-like structures and analysis of SDS solubility.
From: Axin cancer mutants form nanoaggregates to rewire the Wnt signaling network
(a) Quantification of the percentage of Axin-L106R expressing cells carrying an aggresome-like structure. Per field >50 cells were counted. Error bars represent SDs of 7 fields (representing >350 cells) per condition. (b) γ-tubulin staining of cells expressing Axin-wt, Axin-L106R and Axin-L106R F119R reveals that a fraction of Axin-L106R and Axin-L106R F119R (Axin-L106R (F>R) proteins accumulate in a pericentriolar structure. Scale bars 10 μm. (c) Pericentriolar accumulations of Axin-L106R do not display enrichment of HDAC6 or vimentin, in contrast to previously described aggresomes formed by mutant CFTR-ΔF508 protein29. Scale bars 10 μm. (d) Axin-L106R is fully soluble in SDS-based lysis buffer (SDS), similar to the Axin-wt (WT) protein. SDS-resistant pellet fractions (dissolved in formic acid (FA)) were devoid of Axin protein. Actin control is shown for comparison.
