Supplementary Figure 7: Comparison of the IFI values for the five-step and six-step models.
From: Choreography of molecular movements during ribosome progression along mRNA
(a,b) Absolute IFI values for the S13-L33 (red) and S6-L9 (black) FRET pairs. (c,d) Normalized representation of the same data. Note that the trend for the IFI changes is independent of the model. The only difference is the presence of an additional, very rapid step (step 1a or k1a = 300 s−1) in the six-step model (Supplementary Fig. 6b), for which the global fit does not give a statistically significant solution. Average IFI values and error bars are averages with error bars representing standard deviations (s.d.) (n = 3 independent kinetic experiments); in comparison, the s.e.m. of the fit for each IFI is smaller than the symbol size (not shown).
