Supplementary Figure 4: Qualitative and quantitative analysis of OXPHOS subunits from mitochondria-rich tissues of WT and KO male mice.
From: Mammalian elongation factor 4 regulates mitochondrial translation essential for spermatogenesis
(a) Blue native gels (BNG) of heart, liver, muscle and cerebrum mitochondria. (b) In-gel activity (IGA) of complex I and IV. (c) Quantification of IGA from complex I (left) and complex IV (right) (mean ± s.d., n = 4 mice). (d) WB of nuclear and mitochondrial DNA encoded OXPHOS complex subunits. (e) Relative amount (%) of arbitrary units from samples in (d). Tom20: internal control, mean ± s.d., n = 5 mice.
