Supplementary Figure 5: Qualitative and quantitative analysis of OXPHOS complexes and subunits from mitochondria-rich tissues of WT and gKO male mice.
From: Mammalian elongation factor 4 regulates mitochondrial translation essential for spermatogenesis
(a, b) BNG (a) and IGA (b) of heart, liver, muscle and cerebrum mitochondria show similar amount of OXPHOS complexes in WT and gKO mice. (c) Quantification of IGA from complex I (left) and complex IV (right) (mean ± s.d., n = 4 mice). (d) IGA of OXPHOS complexes from WT and gKO heart, testis and epididymis. (e, f) WB of nDNA (e) and mtDNA (f) encoded OXPHOS complex subunits in the samples as in (d). (g, h) Relative amount (%) of arbitrary units from samples in (e) and (f), respectively. H: heart, T: tesits, E: epididymis, Tom20: internal control, mean ± s.d., n = 5 mice, **P < 0.01, ***P < 0.001.
