Supplementary Figure 6: Quantification of mtDNA, mRNA of OXPHOS subunits, and mitochondrial ribosomes.
From: Mammalian elongation factor 4 regulates mitochondrial translation essential for spermatogenesis
(a) Relative mtDNA content (mean ± s.d., n = 10 mice, *P < 0.05). (b) Relative quantification of nDNA (blue) and mtDNA (red) encoded OXPHOS subunits. The ratio was defined as the qPCR value of each tested transcript from KO sample versus from WT sample. Actin: internal control, mean ± s.d., n = 10 mice. (c) Quantification of the expression of mitochondrial transcription factor TFAM (mean ± s.d., n = 5 mice, *P < 0.05). (d) Quantification of the expression of mitochondrial small and large ribosomal subunit protein, MRPS18 and MRPL11, respectively. Tom20: internal control, mean ± s.d., n = 3 mice.
