Supplementary Figure 8: mTOR inhibition in the heart results in OXPHOS defects, and deletion of mtEF4 induces downregulation of cytoplasmic translation in sperm cells.
From: Mammalian elongation factor 4 regulates mitochondrial translation essential for spermatogenesis
(a) Wet weight of the hearts from male mice treated with DMSO or rapamycin (mean ± s.d., n = 5 mice, **P < 0.01). (b) Quantification of the OXPHOS subunits of the DMSO/rapamycin treated mice. The quantitative value of the sample WT heart treated with DMSO was defined as 100%. Tom20: internal control, mean ± s.d., n = 3 mice, *P < 0.05, **P < 0.01. (c, d) Cytoplasmic polysome patterns of WT (blue) and gKO (red) tissues. On the right, the monosomes (80S) versus polysomes ratio was quantified (mean ± s.d., n = 3 mice, *P<0.05). (e, f) Distribution of ATP5D mRNAs across the density gradients from (c, d) was determined by RT-sqPCR. Red square, increased subunits and monosomes in gKO testis than in WT testis.
