Supplementary Figure 1: Generation and validation of mtEF4-knockout mice.
From: Mammalian elongation factor 4 regulates mitochondrial translation essential for spermatogenesis
(a) Alignment of EF4 (E. coli) with mouse, yeast and human EF4. (b) Domain structures of mouse mtEF4 compared to those of EF4 (E. coli). (c) mtEF4 is ubiquitous in mice tissues and organs. (d) Schematic strategy for the generation of mtEF4 KO mouse. (e) Southern blot verification of a single introduction of the targeting construct in the homologous recombined embryonic stem (ES) cell clones. C: control, 129 ES cells. 1-3: positive clones. 5’-: 5’-primer. 3’-: 3’-primer. (f) PCR-based genotyping of KO and gKO mice. (g) qPCR of mtEF4 transcripts from various tissues of WT and KO mice (mean ± s.d., n = 12 mice). (h) Western blotting (WB) of mtEF4 protein from total WT (+/+) and KO (−/−) mice, and gKO mice.
