Supplementary Figure 2: Negative-staining EM and random conical tilt reconstruction of the Ll.LtrB group II intron RNP.
From: Structure of a group II intron in complex with its reverse transcriptase
(a, b) One pair of electron micrographs of an untilted (a) and tilted (b) negatively stained specimen of the group II intron RNP complex. Four tilt pair particles are labeled with colored boxes, one color labeling one molecule. (c) Two-dimensional class averages of the particle images picked from micrographs of untilted specimen. Two major populated class averages are marked in cyan and lavender respectively. (d) The top row shows the RCT 3D reconstruction volumes from the tilted particle images corresponding to the two major class averages shown in (c). The bottom row shows 3D reconstructions obtained by refining the dataset merged from all the untilted and tilted particle images against the two RCT reconstruction volumes, respectively.
