Supplementary Figure 5: C286S mutagenesis does not affect the cell-viability response to serum withdrawal of MCL-1-reconstituted MEFs. | Nature Structural & Molecular Biology

Supplementary Figure 5: C286S mutagenesis does not affect the cell-viability response to serum withdrawal of MCL-1-reconstituted MEFs.

From: Allosteric inhibition of antiapoptotic MCL-1

Supplementary Figure 5

(a) Western blot of lysates from Mcl-1-/- MEFs reconstituted with comparable levels of human, full-length, wild-type or C16S,C286S mutant MCL-1. See also Supplementary Data Set 1. (b) Cell viability (48 h) for the indicated MEFs subjected to serum withdrawal. Data are normalized to the corresponding MEFs maintained in complete media. Error bars are mean ± s.d. (n = 3 technical replicates). *, p <0.01 by two-tailed student’s t test; n.s., not statistically significant.

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