Supplementary Figure 1: Multiple sequence alignments of four Swi2/Snf2 subfamily proteins, ScChd1, SsoRad54 and the RNA helicase Vasa.
From: Structure of chromatin remodeler Swi2/Snf2 in the resting state
The sequence alignments of the Swi2/Snf2 subfamily proteins, ScChd1 and SsoRad54 were done with Clustal Omega, which were further aligned with the sequence of Vasa according to structural superposition (PDB code 2DB3). Secondary structural assignments labeled on the top are based on the structure determined in this study, and the helicase motifs are assigned as reported23. The assignment of HSA domain is based on the reported structure (PDB code 4I6M)20. The SnAc domain is labeled as an orange bar. The residues numbering is based on the sequence of MtSnf2. The suppressor mutants found in ScSth1 are labeled as magenta dots (in postHSA) and yellow dots (in suppH)18. Blue dots, the DNA-binding residues identified in this study; blue triangles, arginine fingers; red triangle, cancer-associated mutations found in human Brg1 gene11,50-53.
50. Bartlett, C., Orvis, T.J., Rosson, G.S. & Weissman, B.E. BRG1 mutations found in human cancer cell lines inactivate Rb-mediated cell-cycle arrest. J. Cell Physiol. 226, 1989-97 (2011).
51. Medina, P.P. et al. Genetic and epigenetic screening for gene alterations of the chromatin-remodeling factor, SMARCA4/BRG1, in lung tumors. Genes Chromosomes Cancer 41, 170-7 (2004).
52. Medina, P.P. et al. Frequent BRG1/SMARCA4-inactivating mutations in human lung cancer cell lines. Hum. Mutat. 29, 617-22 (2008).
53. Wong, A.K. et al. BRG1, a component of the SWI-SNF complex, is mutated in multiple human tumor cell lines. Cancer Res. 60, 6171-7 (2000).
