Supplementary Figure 3: EtBr binding induces minor conformational changes.
From: Lipids modulate the conformational dynamics of a secondary multidrug transporter
DEER distance distributions for spin-labeled cysteine pairs located on the extracellular (top panels) and cytoplasmic (bottom panels) ends of TM helices. No significant changes are observed at pH8 in the absence (black) and in presence of 1mM EtBr (orange), except for the 256R1-310R1 pair, reporting substrate binding. For this pair, substrate binding leads to narrowing of the distance distribution. Targeted helices are highlighted in orange with TM numbers indicated atop, on an LmrP model viewed from the extracellular (top) or cytoplasmic (bottom) side. The N-lobe is blue and the C-lobe is grey.
