Supplementary Figure 4: Uncoupling of the charge-relay network in detergent.
From: Lipids modulate the conformational dynamics of a secondary multidrug transporter
(a) Crystal structure of the LmrP homolog YajR in the outward-open conformation. The charge-relay network of conserved residues is highlighted. TM2 and TM11 are displayed in orange. (b). the single mutations D68N, R72K and D128N were combined with extracellular (160R1-310R1) and (c) cytoplasmic (137R1-349R1) reporters. DEER distance distributions at pH5 (red) pH8 (black) and pH8 + Hoechst 33342 (blue) in the absence (dashed line) and presence (solid line) of each mutation.
