Supplementary Figure 1: Purification and negative-stain EM of K. lactis Cog5–8. | Nature Structural & Molecular Biology

Supplementary Figure 1: Purification and negative-stain EM of K. lactis Cog5–8.

From: Molecular architecture of the complete COG tethering complex

Supplementary Figure 1

(a) The purified recombinant K. lactis Cog5-8 complex, visualized by SDS-PAGE and Coomassie Blue staining, is shown together with a representative image field and ten representative class averages. (b) Complete gallery of K. lactis Cog5-8 class averages (side length of each panel = 38 nm). The 129 class averages, which account for 4,958 particles, were obtained by subjecting 8,825 particles to 14 generations of ISAC.

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