Supplementary Figure 3: Localizing individual subunits within the K. lactis Cog5–8 complex.
From: Molecular architecture of the complete COG tethering complex
(a) Purified K. lactis Cog5-8 complexes containing single GFP tags, visualized by SDS-PAGE and Coomassie Blue staining. GFP-Cog6ΔN denotes N-terminally truncated Cog6 (residues 147-779). (b) Complete galleries of GFP-tagged K. lactis Cog5-8 class averages (side length of each panel = 38 nm). Only those complexes in which the GFP tag was visible in class averages are included; the remaining complexes were indistinguishable from the untagged complexes shown in Supplementary Fig. 1b.
